4. Assemblies of the bumble bee, Bombus impatiens (estimated size 250 Mbp). E-size is a statistic calculated on contigs and scaffolds and is designed to answer the question: if you choose a location (a base) in the reference genome at random, what is the expected size of the contig or scaffold containing that location? E-size is computed as: ∑G=(LC)2/G,where LC is the length of contig C, and G is the genome length estimated by the sum of all contig lengths.

Assembler

Contigs

Scaffolds

Num

N50

(kb)

E-size

(kb)

Num

N50

(kb)

E-size

(kb)

ABySS

40,451

14.4

20.9

34,298

18

27

Allpaths-LG

Could not run: incompatible library types

CABOG

22,107

23.5

34.2

1,191

1,125

1,367

MSR-CA

21,885

32.4

46.9

2,551

1,246

1,528

SGA

Program crashed: cause unclear

SOAPdenovo

15,957

57.1

78.2

5,800

1,374

1,608

Velvet

Program crashed: insufficient memory (256 GB)